High-fat, high-carbohydrate diet-induced experimental model of metabolic syndrome in rats

Purpose. The study is focused on development of high-fat, high-carbohydrate diet-induced experimental model metabolic syndrome (MS) in rats. Materials and methods. 6-week old Wistar rats ( n = 20) were used for study. Rats separated into control groups. from the group fed standard rat chow. had a diet rich lard (17%) fructose drank 20% solution. At end study, body weight blood pressure (BP) assessed. After 12 weeks load, an oral glucose tolerance test (GTT) insulin (ITT) carried out. Lipid protein biochemical parameters plasma analyzed. Adipose tissue liver measured at levels triacylglycerol (TAG) cholesterol (Сh) determined by enzymatic Results. High-fat, feeding led to BP elevation increase adipose tissue/body ratio. Hyperglycemia, impaired resistance found with MS means GTT ITT. Elevation TAG level was observed group, although total Сh HDL-Ch did not differ those controls. Liver/body ratio Ch elevated MS. Conclusion. Experimental reproduces many aspects humans. This may be useful studying pathophysiology methods its prevention treatment.